Plin2^tm1Chan
Targeted Allele Detail

Summary

• Symbol: Plin2^tm1Chan
• Name: perilipin 2; targeted mutation 1, Lawrence Chan
• MGI ID: MGI:3617784
• Synonyms: Adfp^-, delta2,3 ADPH
• Gene: Plin2 Location: Chr4:86566623-86588297 bp, - strand Genetic Position: Chr4, 40.69 cM
• Alliance: Plin2^tm1Chan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:105572
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: Homologous recombination was used to replace exons 2 and 3 with a beta geo cassette. Northern and Western blot analyses failed to detect transcript and protein, respectively, in mutants. However, a subsequent study has identified a truncated translation product beginning at an alternative start site in exon 5 by using a C-terminal specific antibody. The truncated protein is detectable in cytoplasmic lipid droplets of the secretory epithelial cells in mammary glands after parturition. (J:105572, J:130099)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plin2 Mutation: 37 strains or lines available

References

• Original: J:105572 Chang BH, et al., Protection against fatty liver but normal adipogenesis in mice lacking adipose differentiation-related protein. Mol Cell Biol. 2006 Feb;26(3):1063-76
• All: 11 reference(s)