Rb1^tm1Dwg
Targeted Allele Detail

Summary

• Symbol: Rb1^tm1Dwg
• Name: RB transcriptional corepressor 1; targeted mutation 1, David W Goodrich
• MGI ID: MGI:3617652
• Synonyms: Rb1⁶⁵⁴, Rb R654W
• Gene: Rb1 Location: Chr14:73430298-73563446 bp, - strand Genetic Position: Chr14, 38.73 cM, cytoband D3
• Alliance: Rb1^tm1Dwg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:105548
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Hypomorph, Modified isoform(s))
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Arginine codon 654 (CGA) exon 20 was changed to tryptophan (TGG) (p.R654W). The loxP site flanked neomycin resistance gene cassette that was inserted into intron 19 was removed via Cre-mediated recombination. Western blot demonstrated protein levels were approximately the same in mutants and wild-type. (J:105548)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	9 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Rb1^tm1Dwg

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rb1 Mutation: 106 strains or lines available

References

• Original: J:105548 Sun H, et al., An E2F binding-deficient Rb1 protein partially rescues developmental defects associated with Rb1 nullizygosity. Mol Cell Biol. 2006 Feb;26(4):1527-37
• All: 4 reference(s)