Polk^tm1Hohm
Targeted Allele Detail

Summary

• Symbol: Polk^tm1Hohm
• Name: polymerase (DNA directed), kappa; targeted mutation 1, Haruo Ohmori
• MGI ID: MGI:3615272
• Synonyms: Polk^-, Polk^tm1Taz
• Gene: Polk Location: Chr13:96617198-96679087 bp, - strand Genetic Position: Chr13, 50.56 cM, cytoband D1
• Alliance: Polk^tm1Hohm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83202
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exon 5 with a PGK-neo cassette. Mutant ES cells failed to express protein. (J:91863)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Polk Mutation: 65 strains or lines available

Notes

Homozygous ES cells grow normally, however they slightly more sensitive to UV and x-ray radiation and are hypersensitive to both killing and mutagenesis induced by benzo[a]pyrene (B[a]P) (J:91863).

References

• Original: J:83202 Shimizu T, et al., The absence of DNA polymerase kappa does not affect somatic hypermutation of the mouse immunoglobulin heavy chain gene. Immunol Lett. 2003 May 1;86(3):265-70
• All: 3 reference(s)