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Targeted Allele Detail
Symbol: Hif1atm1.1Mcs
Name: hypoxia inducible factor 1, alpha subunit; targeted mutation 1.1, M Celeste Simon
MGI ID: MGI:3613538
Synonyms: Hif-1alpha KI
Gene: Hif1a  Location: Chr12:73901375-73947530 bp, + strand  Genetic Position: Chr12, 31.99 cM
Germline Transmission:  Earliest citation of germline transmission: J:97164
Parent Cell Line:  TL1/TL-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Inserted expressed sequence, Null/knockout)
Mutation:    Insertion
Mutation detailsCre mediated recombination removed a floxed neo, leaving exon 1, containing the ATG, replaced by a c-Myc epitope tagged Epas1 cDNA such that knock-in expression depended upon the endogenous regulatory sequence. Mutant ES cells did not express Hif1a protein, as shown by Western blot and EMSA analyses. Immunoprecipitation and immunoblot detected expression of the knock-in sequence. (J:97164)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hif1a Mutation:  11 strains or lines available
Injection of homozygous ES cells into nude mice promotes faster growth of larger, more hemorrhagic tumors than wild-type ES cells. These tumors have increased microvessel density and a wider range of differentiated cell types than tumors from wild-type cells (J:97164). Homozygous ES clones form significantly fewer CFUs (hematopoietic colony-forming units) of all hematopoietic lineages and form 7.8-fold more secondary embryoid bodies (Ebs) compared with wild-type, consistent with maintenance of an undifferentiated state (J:105812).
Original:  J:97164 Covello KL, et al., Targeted replacement of hypoxia-inducible factor-1alpha by a hypoxia-inducible factor-2alpha knock-in allele promotes tumor growth. Cancer Res. 2005 Mar 15;65(6):2277-86
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.15
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