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Braftm1Cpri
Targeted Allele Detail
Nomenclature
Symbol: Braftm1Cpri
Name: Braf transforming gene; targeted mutation 1, Catrin Pritchard
MGI ID: MGI:3612533
Synonyms: LSL-B-rafV600E, LSL-BRAFV600E
Gene: Braf  Location: Chr6:39603237-39725463 bp, - strand  Genetic Position: Chr6, 18.43 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:104375
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutations:    Insertion, Single point mutation
 
Mutation detailsA targeting vector was designed to insert a T1799A mutation into exon 15 and a loxP-stop-loxP cassette containing a minigene encoding exons 15-18 of wild-type Braf with a splice acceptor sequence at the 5' end into intron 14. Upon cre expression, the loxP-stop-loxP is deleted, enabling expression of the knock-in sequence. This mutation increases the kinase activity of the protein. (J:104375)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Braf Mutation:  20 strains or lines available
Notes
Phenotypic Similarity to Human Syndrome: Melanoma (tamoxifen-treated Braftm1Cpri/Braftm1Cpri Tg(Tyr-cre/ERT2)1Laru mice J:147434)

References
Original:  J:104375 Mercer K, et al., Expression of endogenous oncogenic V600EB-raf induces proliferation and developmental defects in mice and transformation of primary fibroblasts. Cancer Res. 2005 Dec 15;65(24):11493-500
All:  9 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
01/13/2015
MGI 5.21
The Jackson Laboratory