Rasgrf1^tm2Pds
Targeted Allele Detail

Summary

• Symbol: Rasgrf1^tm2Pds
• Name: RAS protein-specific guanine nucleotide-releasing factor 1; targeted mutation 2, Paul D Soloway
• MGI ID: MGI:3611766
• Synonyms: Ras-GRF1 OE
• Gene: Rasgrf1 Location: Chr9:89791961-89909030 bp, + strand Genetic Position: Chr9, 47.31 cM
• Alliance: Rasgrf1^tm2Pds page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103751
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The pBJR2 vector was used to insert a 1.5-kb neo cassette and a 500-nt Pgk enhancer and promoter fragment in reverse orientation such that it replaced the Rasgrf1 repeats located downstream of the differentially methylated domain. Removal of the paternal repeats caused a loss of paternal allele methylation as shown by Southern blot. The enhancer insertion enabled expression of the transcript whether it was transmitted maternally or paternally. Expression was due entirely to the presence of the Pgk-enhancer and promoter insertion and not due to a repeat deletion. (J:103751, J:185711)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rasgrf1 Mutation: 65 strains or lines available

References

• Original: J:103751 Yoon B, et al., Rasgrf1 imprinting is regulated by a CTCF-dependent methylation-sensitive enhancer blocker. Mol Cell Biol. 2005 Dec;25(24):11184-90
• All: 5 reference(s)