Casp8^tm1Syon
Targeted Allele Detail

Summary

• Symbol: Casp8^tm1Syon
• Name: caspase 8; targeted mutation 1, S Yonehara
• MGI ID: MGI:3609638
• Synonyms: caspase-8^DED
• Gene: Casp8 Location: Chr1:58834533-58886662 bp, + strand Genetic Position: Chr1, 29.19 cM, cytoband B
• Alliance: Casp8^tm1Syon page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103506
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A stop codon, SV40 polyA sequence and a pgk-neo cassette was inserted into exon 7 via homologous recombination. RT-PCR of mutants detected a truncated transcript. Immunoblot analysis detected a peptide at 25 kDa corresponding to the DED domains of the gene. (J:103506)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Casp8 Mutation: 42 strains or lines available

References

• Original: J:103506 Sakamaki K, et al., Ex vivo whole-embryo culture of caspase-8-deficient embryos normalize their aberrant phenotypes in the developing neural tube and heart. Cell Death Differ. 2002 Nov;9(11):1196-206
• All: 5 reference(s)