Gt(ROSA)26Sor^{tm1(rtTA-IFP1)Miya}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(rtTA-IFP1)Miya}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Jun-ichi Miyazaki
• MGI ID: MGI:3609482
• Synonyms: RTF-pdx-1-EGFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(rtTA-IFP1)Miya} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:102833
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter, Transactivator)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(rtTA-IFP1)Miya} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pdx1 (MGI:102851)

• Mutation details: EB3 ES cells were targeted with a vector containing the Tet-off regulation cassette for the homeodomain transcription factor Ipf1 and EGFP expression. A floxed neo was removed via cre-mediated excision. EB3 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14Tg2a ES cells with an IRESBSDpA selection cassette. (J:102833, J:103479)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

Notes

ES cell line = EB3, a mutation of ES cell line E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette.

References

• Original: J:102833 Miyazaki S, et al., Development of a single-cassette system for spatiotemporal gene regulation in mice. Biochem Biophys Res Commun. 2005 Dec 16;338(2):1083-8
• All: 4 reference(s)