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Transgene Detail
Symbol: Tg(Ins2-GAD2)1Lt
Name: transgene insertion 1, Edward H Leiter
MGI ID: MGI:3606176
Synonyms: RIP7-hGAD65, TgN(RIP7hGAD65)Lt4 Y-line
Transgene: Tg(Ins2-GAD2)1Lt  Location: unknown  Genetic Position: ChrY, Syntenic
Strain of Origin:  NOD/ShiLt
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
Tg(Ins2-GAD2)1Lt expresses 1 gene
Mutation detailsThe transgene comprises the cDNA sequence encoding human glutamic acid decarboxylase 2 under control of a modified rat insulin 2 promoter composed of 9.5 kb of genomic DNA from upstream of the transcription initiation site fused to the first intron of Ins2. Transmission from the founder male only to male progeny demonstrated integration into the Y chromosome. Densitrometric Southern blot analysis indicates presence of approximately 17 copies of the transgene. Expression in the pancreatic islets of transgenic mRNA has been demonstrated by reverse transcription followed by PCR (RT-PCR), and of GAD2 protein by western blot analysis. (J:100251)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Original:  J:100251 Bridgett M, et al., Differential protection in two transgenic lines of NOD/Lt mice hyperexpressing the autoantigen GAD65 in pancreatic beta-cells. Diabetes. 1998 Dec;47(12):1848-56
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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