Rho^tm2Jlem
Targeted Allele Detail

Summary

• Symbol: Rho^tm2Jlem
• Name: rhodopsin; targeted mutation 2, Janis Lem
• MGI ID: MGI:3590580
• Synonyms: Palm^-
• Gene: Rho Location: Chr6:115908709-115916997 bp, + strand Genetic Position: Chr6, 53.72 cM
• Alliance: Rho^tm2Jlem page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100845
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting construct was designed to insert Cys322Thr and Cys323Ser modifications in the locus to eliminate both palmitoylation sites. A tACE-Cre-neo cassette was included in the construct and self-excised upon passage through the male germ line. Expression of palmitoylation-deficient rhodopsin transcript was demonstrated by RT-PCR, and the absence of palmitates on rhodopsin protein of mutant mice was confirmed by mass spectrometry. (J:100845)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rho Mutation: 48 strains or lines available

References

• Original: J:100845 Wang Z, et al., Enhanced shutoff of phototransduction in transgenic mice expressing palmitoylation-deficient rhodopsin. J Biol Chem. 2005 Jul 1;280(26):24293-300
• All: 4 reference(s)