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Targeted Allele Detail
Symbol: Rhotm2Jlem
Name: rhodopsin; targeted mutation 2, Janis Lem
MGI ID: MGI:3590580
Synonyms: Palm-
Gene: Rho  Location: Chr6:115931748-115940036 bp, + strand  Genetic Position: Chr6, 53.72 cM
Germline Transmission:  Earliest citation of germline transmission: J:100845
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Nucleotide substitutions
Mutation detailsA targeting construct was designed to insert Cys322Thr and Cys323Ser modifications in the locus to eliminate both palmitoylation sites. A tACE-Cre-neo cassette was included in the construct and self-excised upon passage through the male germ line. Expression of palmitoylation-deficient rhodopsin transcript was demonstrated by RT-PCR, and the absence of palmitates on rhodopsin protein of mutant mice was confirmed by mass spectrometry. (J:100845)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rho Mutation:  13 strains or lines available
Original:  J:100845 Wang Z, et al., Enhanced shutoff of phototransduction in transgenic mice expressing palmitoylation-deficient rhodopsin. J Biol Chem. 2005 Jul 1;280(26):24293-300
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.15
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