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Transgene Detail
Symbol: Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell
Name: transgene insertion 1, John F Elliott
MGI ID: MGI:3590082
Synonyms: DQ8+, human CD4, DQ8 transgene, human CD4, DQA1*0301, DQB1*0302 transgene, Tg(CD2-CD4,HLA-DQA1*0301,HLA-DQB1*0302)1Ell, Tg(CD4,HLA-DQ8)1Ell
Transgene: Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell  Location: unknown  
Strain of Origin:  NOD/MrkTac
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell expresses 3 genes
Mutation detailsFertilized oocytes were co-injected with two DNA fragments: a human CD4 cDNA joined to the promoter from the human CD2 gene and a large DNA fragment, reassembled from segments of the cosmid pDCbeta2, containing the human major histocompatibility (MHC) class II alleles HLA-DQA1*0301 and HLA-DQB*0302, which together encode the dimer comprising the HLA-DQ8 molecule, plus 5' regulatory elements. Human CD4 is expressed at a low level on all T cells of transgenic mice. HLA-DQ8 is expressed in homozygous NOD mice at a level comparable to that of the endogenous NOD H2-Abetag7 MHC class II molecule in wild-type NOD mice. (J:87013, J:113267)
View phenotypes for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Although in NOD mice homozygous for this transgene alone endogeous NOD H2-Abetag7 and transgene-derived HLA-DQ8 are expressed at similar levels, virtually all CD4+ T-cell responses are H2Abeta1g7 restricted (J:87013).

Phenotypic Similarity to Human Syndrome: Idiopathic Dilated Cardiomyopathy in double homozygous mice of the strain NOD.Cg-H2-Ab1tm1Gru Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell (J:86540)

Original:  J:87013 Liu J, et al., Major DQ8-restricted T-cell epitopes for human GAD65 mapped using human CD4, DQA1*0301, DQB1*0302 transgenic IA(null) NOD mice. Diabetes. 1999 Mar;48(3):469-77
All:  3 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.07
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