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Targeted Allele Detail
Symbol: Gt(ROSA)26Sortm1.1(birA)Mejr
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Dies Meijer
MGI ID: MGI:3588507
Synonyms: bira, Rosa26BirA
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Germline Transmission:  Earliest citation of germline transmission: J:100227
Parent Cell Line:  IB10/E14IB10 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Not Applicable)
Mutation:    Insertion
Mutation detailsAn HA (hemagglutinin) tagged E. coli bacterial birA, bifunctional protein (biotin ligase), gene was inserted into exon 3 of the rabbit beta-globin gene. The entire birA ORF, with the rabbit beta globin splice acceptor and transcription termination sequences were excised to generate a targeting vector that also contained a floxed PGK- puromycin selection cassette. This targeting vector was inserted into the first intron of the Gt(ROSA)26Sor gene. Cre-mediated recombination removed the floxed puromycin cassette. Western blot of mutants demonstrated the expression of birA in all tissues tested, though levels of expression varied from tissue to tissue. (J:100227)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 10 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  572 strains or lines available
Original:  J:100227 Driegen S, et al., A generic tool for biotinylation of tagged proteins in transgenic mice. Transgenic Res. 2005 Aug;14(4):477-82
All:  20 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
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