Onecut2^tm1Flem
Targeted Allele Detail

Summary

• Symbol: Onecut2^tm1Flem
• Name: one cut domain, family member 2; targeted mutation 1, Frederic P Lemaigre
• MGI ID: MGI:3588425
• Synonyms: Oc2^-, Oc2^lacZ
• Gene: Onecut2 Location: Chr18:64473098-64531559 bp, + strand Genetic Position: Chr18, 37.33 cM, cytoband D3
• Alliance: Onecut2^tm1Flem page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100352
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was designed to insert a lacZ-puro cassette into exon 1, deleting sequence that encodes transcriptional regulatory regions and for the CUT domain required for DNA binding. In-frame stop codons were included in the vector, following the lacZ sequence to prevent the production of a truncated Onecut2 protein or read-through transcripts initiated in the lacZ or puro cassettes. RT-PCR failed to detect Onecut2 transcript in mutant liver samples. (J:100352)

Expression

In Mice Carrying this Mutation:	100 assay results 3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Onecut2 Mutation: 20 strains or lines available

References

• Original: J:100352 Clotman F, et al., Control of liver cell fate decision by a gradient of TGFbeta signaling modulated by Onecut transcription factors. Genes Dev. 2005 Aug 15;19(16):1849-54
• All: 18 reference(s)