Tg(HLA-DQA1,HLA-DQB1)1Dv
Transgene Detail

Summary

• Symbol: Tg(HLA-DQA1,HLA-DQB1)1Dv
• Name: transgene insertion 1, Chella David
• MGI ID: MGI:3586633
• Synonyms: Abeta⁰.DQ8, DQ8, HLA-DQ8, HLA-DQ8 Tg, HLA-DQA1*0301/DQB1*0302, Tg(HLA-DQA1*301,HLA-DQB1*302)1Dv
• Transgene: Tg(HLA-DQA1,HLA-DQB1)1Dv Location: unknown
• Alliance: Tg(HLA-DQA1,HLA-DQB1)1Dv page

Transgene origin

• Strain of Origin: (CBA/J x B10.M-H2^f)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A 30-kb human genomic DNA fragment containing the entire HLA-DQA1*0301 gene and a promoter-truncated HLA-DQB1*0302 gene from cosmid H11A and a 38-kb DNA fragment containing the entire HLA-DQB1*0302 gene from cosmid X10A were coinjected. (J:88296)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

Notes

Flow cytometric analysis of peripheral blood leukocytes (PBL) using a monoclonal antibody to HLA-DQ demonstrated that transgenic mice express the transgenes. 25-40% of the PBLs of mixed-background transgenic mice homozygous for the targeted MHC class II mutation H2-Ab1^tm1Dim express the transgenes. Lymph nodes of such mice contain three-fold the levels of CD4⁺ CD44⁺ T-cells as do those of nontransgenic MHC class II-deficient mice, levels similar to those of class II-sufficient mice. Immunization of transgenic H2-Ab1^tm1Dim homozygous mice with bovine collagen II results in a strong IgG antibody response to the immunogen, and 66% of these mice develop severe, persistent arthritis. (J:88296)

References

• Original: J:88296 Nabozny GH, et al., HLA-DQ8 transgenic mice are highly susceptible to collagen-induced arthritis: a novel model for human polyarthritis. J Exp Med. 1996 Jan 1;183(1):27-37
• All: 71 reference(s)