Tg(Ins2-OVA)307Wehi
Transgene Detail

Summary

• Symbol: Tg(Ins2-OVA)307Wehi
• Name: transgene insertion 307, Walter and Eliza Hall Institute of Medical Research
• MGI ID: MGI:3583231
• Synonyms: RIP-OVA, RIP-OVA^lo
• Transgene: Tg(Ins2-OVA)307Wehi Location: unknown
• Alliance: Tg(Ins2-OVA)307Wehi page

Transgene origin

• Strain of Origin: B6.C-H2^bm1/ByJWehi

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-OVA)307Wehi expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  chicken	OVAL (396058)

• Mutation details: This transgene expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA under control of the rat insulin 2 promoter. (J:99451)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Immunohistochemical analysis does not detect ovalbumin (OV) in the pancreatic islet beta cells of mice bearing this transgene, nor does western blot analysis, indicating that their OV content is less than 0.03 ng/ug of protein. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an ovalbumin-specific T cell receptor expressed on CD8+ T lymphocytes, develop early onset, spontaneous diabetes with islet infiltration, implying that the beta cells express OV. While only one of 60 lethally irradiated Tg(Ins2-OV)307Wehi mice receiving a 1:4 (OT-1 mouse:C57BL/6-Thy1^a congenic) bone marrow mixture became diabetic during the 10 weeks post transfer, histological evaluation of these mice at 15-21 weeks post-transfer showed mild infiltration of 22% of their islets.

References

• Original: J:99451 Blanas E, et al., Induction of autoimmune diabetes by oral administration of autoantigen. Science. 1996 Dec 6;274(5293):1707-9
• All: 17 reference(s)