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Tg(Ins2-OVA)307Wehi
Transgene Detail
Nomenclature
Symbol: Tg(Ins2-OVA)307Wehi
Name: transgene insertion 307, Walter and Eliza Hall Institute of Medical Research
MGI ID: MGI:3583231
Synonyms: RIP-OVA, RIP-OVAlo
Transgene: Tg(Ins2-OVA)307Wehi  Location: unknown  
Transgene
origin
Strain of Origin:  B6.C-H2bm1/ByJWehi
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(Ins2-OVA)307Wehi expresses 1 gene
 
Mutation detailsThis transgene expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA under control of the rat insulin 2 promoter. (J:99451)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
Immunohistochemical analysis does not detect ovalbumin (OV) in the pancreatic islet beta cells of mice bearing this transgene, nor does western blot analysis, indicating that their OV content is less than 0.03 ng/ug of protein. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an ovalbumin-specific T cell receptor expressed on CD8+ T lymphocytes, develop early onset, spontaneous diabetes with islet infiltration, implying that the beta cells express OV. While only one of 60 lethally irradiated Tg(Ins2-OV)307Wehi mice receiving a 1:4 (OT-1 mouse:C57BL/6-Thy1a congenic) bone marrow mixture became diabetic during the 10 weeks post transfer, histological evaluation of these mice at 15-21 weeks post-transfer showed mild infiltration of 22% of their islets.
References
Original:  J:99451 Blanas E, et al., Induction of autoimmune diabetes by oral administration of autoantigen. Science. 1996 Dec 6;274(5293):1707-9
All:  16 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
04/06/2021
MGI 6.16
The Jackson Laboratory