About   Help   FAQ
Transgene Detail
Symbol: Tg(Ins2-OVA)307Wehi
Name: transgene insertion 307, Walter and Eliza Hall Institute of Medical Research
MGI ID: MGI:3583231
Synonyms: RIP-OVA, RIP-OVAlo
Transgene: Tg(Ins2-OVA)307Wehi  Location: unknown  
Strain of Origin:  B6.C-H2bm1/ByJWehi
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(Ins2-OVA)307Wehi expresses 1 gene
Mutation detailsThis transgene expresses a secreted form of ovalbumin from the full-length chicken ovalbumin cDNA under control of the rat insulin 2 promoter. (J:99451)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Immunohistochemical analysis does not detect ovalbumin (OV) in the pancreatic islet beta cells of mice bearing this transgene, nor does western blot analysis, indicating that their OV content is less than 0.03 ng/ug of protein. Mice expressing both this transgene and Tg(Tcra,Tcrb)1100Mjb (OT-1), which encodes an ovalbumin-specific T cell receptor expressed on CD8+ T lymphocytes, develop early onset, spontaneous diabetes with islet infiltration, implying that the beta cells express OV. While only one of 60 lethally irradiated Tg(Ins2-OV)307Wehi mice receiving a 1:4 (OT-1 mouse:C57BL/6-Thy1a congenic) bone marrow mixture became diabetic during the 10 weeks post transfer, histological evaluation of these mice at 15-21 weeks post-transfer showed mild infiltration of 22% of their islets.
Original:  J:99451 Blanas E, et al., Induction of autoimmune diabetes by oral administration of autoantigen. Science. 1996 Dec 6;274(5293):1707-9
All:  16 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
MGI 6.16
The Jackson Laboratory