Hprt1^{tm2(Pgk1-Pac/TK)Brd}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm2(Pgk1-Pac/TK)Brd}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 2, Allan Bradley
• MGI ID: MGI:3582838
• Synonyms: puDeltatk
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm2(Pgk1-Pac/TK)Brd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99258
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The coding sequence of the transgene consists of the puromycin acetyltransferase coding sequence fused in-frame to a 5' truncated HSV1 thymidine kinase gene that begins at the second ATG of the full-length gene and encodes a protein lacking the carboxy-terminal 45 amino acids. This construct resides upstream of the bovine growth hormone polyadenylation signal and downstream of the phosphoglycerate kinase 1 promoter followed by a loxP-flanked neomycin acetyltransferase gene with the bovine growth hormone poly-A signal. The transgene was inserted in reverse orientation into exon 3 of the endogenous Hprt gene via homologous recombination. (J:99258, J:99558)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1273 strains or lines available

Notes

When mice bearing this transgene are crossed with mice transgenic for cre, excision of the loxP-flanked neo gene in cells expressing Cre recombinase results in expression of a puromycin acetyltransferase-thymidine kinase fusion protein under control of the (ubiquitously-expressing) Pgk1 promoter. Treatment of the mice with ganciclovir (GCV) causes specific ablation of cells expressing HSV1 Tk activity.

References

• Original: J:99258 Chen YT, et al., Bigenic Cre/loxP, puDeltatk conditional genetic ablation. Nucleic Acids Res. 2004;32(20):e161
• All: 5 reference(s)