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Targeted Allele Detail
Symbol: Sema5atm1Awp
Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; targeted mutation 1, Andreas W Puschel
MGI ID: MGI:3577050
Synonyms: Sema5a-
Gene: Sema5a  Location: Chr15:32244959-32696487 bp, + strand  Genetic Position: Chr15, 13.02 cM
Germline Transmission:  Earliest citation of germline transmission: J:97663
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA targeting vector was designed to replace exons 4 and 5 with a PGK-neo cassette. The null allele was achieved by inserting a stop codon after amino acid 121, thereby rendering a truncated protein unlikely to be biological active. Alternatively, aberrant splicing between exons 3 and 6 would result in the generation of a stop codon after amino acid 141. Western blot analysis of membrane fractions demonstrated a complete lack of protein in mutants. (J:97663)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Sema5a Mutation:  43 strains or lines available
Original:  J:97663 Fiore R, et al., Inactivation of the Sema5a gene results in embryonic lethality and defective remodeling of the cranial vascular system. Mol Cell Biol. 2005 Mar;25(6):2310-9
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory