Tg(Pcp2/Jun)18Dcrl
Transgene Detail

Summary

• Symbol: Tg(Pcp2/Jun)18Dcrl
• Name: transgene insertion 18, Daniella Carulli
• MGI ID: MGI:3573864
• Synonyms: L7-c-jun, L7-c-jun-Tg, Pcp2(L7) promoter/c-jun
• Transgene: Tg(Pcp2/Jun)18Dcrl Location: unknown
• Alliance: Tg(Pcp2/Jun)18Dcrl page

Transgene origin

• Strain of Origin: FVB/NIco

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Pcp2/Jun)18Dcrl expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Jun (MGI:96646)

• Mutation details: The Jun oncogene cDNA was inserted into the L7deltaAUG vector, which is a PCR-mutated Purkinje cell protein 2 gene. The vector comprises the entire genomic sequence of Pcp2, including 1 kilobase of 5'-flanking sequence and about 200 bp of 3'-flanking sequence, modified to eliminate a BamHI restriction site in the third intron and all potential transcription start sites. The cDNA was inserted into the only remaining BamHI site, in exon 4, so transcription occurs from the Pcp2 promoter and translation from the initiation site of the cDNA. (J:96313, J:96314)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

This was one of four transgenic lines (18, 21, 35 and 61) immunostaining of whose cerebella demonstrated similar, high levels of JUN expression in Purkinje cell nuclei.

References

• Original: J:96314 Carulli D, et al., Regenerative and survival capabilities of Purkinje cells overexpressing c-Jun. Eur J Neurosci. 2002 Jul;16(1):105-18
• All: 2 reference(s)