Tg(Rr28-Igf2)10bDdp
Transgene Detail

Summary

• Symbol: Tg(Rr28-Igf2)10bDdp
• Name: transgene insertion 10b, Dimitrina D Pravtcheva
• MGI ID: MGI:3573690
• Synonyms: 10B, pmH19eIgfMlu 10B
• Transgene: Tg(Rr28-Igf2)10bDdp Location: unknown
• Alliance: Tg(Rr28-Igf2)10bDdp page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Rr28-Igf2)10bDdp expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Igf2 (MGI:96434)

• Mutation details: The transgene contains a 17 kb genomic fragment containing the entire coding region of a partially methylated mouse Igf2 under control of its own promoter. A small 5' fragment of the noncoding region of exon 1 was left out of this construct. The MluI restriction site at the 3' end of the nontranslated portion of exon 6 was abolished to distinguish the transgene from the endogenous Igf2 gene.A 7 kb genomic fragment containing the mouse Igf2/H19 enhancer sequences (Rr28) from downstream of H19 was cloned upstream of the Igf2 gene. RT-PCR analysis showed transgene expression equal to or slightly higher than the endogenous Igf2 protein in the liver, kidney, lung, heart, and mammary tissue. (J:90325)

Tumor Data

List all tumor models in MMHCdb carrying Tg(Rr28-Igf2)10bDdp

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:90325 Wise TL, et al., Perinatal lethality in H19 enhancers-Igf2 transgenic mice. Mol Reprod Dev. 1997 Oct;48(2):194-207
• All: 2 reference(s)