Tg(Th-SNCA*A53T)1Ddim
Transgene Detail

Summary

• Symbol: Tg(Th-SNCA*A53T)1Ddim
• Name: transgene insertion 1, Donato A Di Monte
• MGI ID: MGI:3529023
• Synonyms: Tg(Thy1-SNCA*A53T)1Ddim, TH-A53T
• Transgene: Tg(Th-SNCA*A53T)1Ddim Location: unknown
• Alliance: Tg(Th-SNCA*A53T)1Ddim page

Transgene origin

• Strain of Origin: Swiss Webster x (C57BL/6 x DBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Th-SNCA*A53T)1Ddim expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SNCA (6622)

  Snca	(MGI:1277151)

  Transgene codes for a protein with an alanine to threonine mutation.

• Tg(Th-SNCA*A53T)1Ddim expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Th (25085)

  Th	(MGI:98735)

• Mutation details: The transgene contains 4.8 kb of rat tyrosine hydroxylase (Thy1) promoter sequence, a human alpha synuclein (SNCA) cDNA sequence carrying a G209A transition mutation, which encodes an Ala53Thr amino acid substitution, and an SV40 polyadenylation sequence. (J:70209)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Six transgenic lines were generated from the A53T construct. The transgene is expressed within nigrostriatal dopaminergic neurons. A similar and consistent pattern of transgene expression in all lines of transgenic mice was revealed by in situ hybridization studies. Transgenic protein accumulation impacted neither the number of nigral neurons, nor the levels of striatal dopamine in animals up to one year of age. No overt nuerodegerative changes were detected in transgenic mice.

References

• Original: J:70209 Matsuoka Y, et al., Lack of nigral pathology in transgenic mice expressing human alpha-synuclein driven by the tyrosine hydroxylase promoter. Neurobiol Dis. 2001 Jun;8(3):535-9
• All: 3 reference(s)