Stat2^tm1Jdf
Targeted Allele Detail

Summary

• Symbol: Stat2^tm1Jdf
• Name: signal transducer and activator of transcription 2; targeted mutation 1, J David Farrar
• MGI ID: MGI:3528233
• Synonyms: M/H Stat2
• Gene: Stat2 Location: Chr10:128106428-128128718 bp, + strand Genetic Position: Chr10, 76.5 cM
• Alliance: Stat2^tm1Jdf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95855
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A sequence within exon 23 beginning with nucleotide 51 was replaced with the corresponding human cDNA encoding the C terminus of the gene and a stop codon. A loxP flanked neo was inserted into intron 23 and subsequently deleted via transient cre expression, leaving a single loxP site. Immunoprecipitation and Western blotting confirmed successful targeting and the presence of a chimeric mouse-human protein in mutants. (J:95855)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stat2 Mutation: 35 strains or lines available

References

• Original: J:95855 Persky ME, et al., IL-12, but not IFN-alpha, promotes STAT4 activation and Th1 development in murine CD4+ T cells expressing a chimeric murine/human Stat2 gene. J Immunol. 2005 Jan 1;174(1):294-301
• All: 2 reference(s)