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Flt1tm2Msh
Targeted Allele Detail
Nomenclature
Symbol: Flt1tm2Msh
Name: FMS-like tyrosine kinase 1; targeted mutation 2, Masabumi Shibuya
MGI ID: MGI:3527129
Synonyms: Flt-TK-, VEGFR1(TM-TK)-
Gene: Flt1  Location: Chr5:147561604-147726011 bp, - strand  Genetic Position: Chr5, 87.01 cM, cytoband G
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:95387
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA neomycin resistance gene replaced exon 16, encoding the transmembrane domain. No clear bands were seen for exons downstream of exon 15 in RT-PCR analysis, showing that the protein was truncated at the termination codon in exon 15. Mutant lung tissues demonstrated that the 110-kDa endogenous soluble form was present, as it results from a termination in intron 13. Also present was a new truncated form, approximately 120-kDa, detected with an antiserum raised against the amino acid sequence of exon 15. (J:95387)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Flt1 Mutation:  42 strains or lines available
References
Original:  J:95387 Hiratsuka S, et al., Membrane fixation of vascular endothelial growth factor receptor 1 ligand-binding domain is important for vasculogenesis and angiogenesis in mice. Mol Cell Biol. 2005 Jan;25(1):346-54
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
11/14/2017
MGI 6.11
The Jackson Laboratory