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Transgene Detail
Symbol: Tg(APPswe,PSEN1dE9)85Dbo
Name: transgene insertion 85, David R Borchelt
MGI ID: MGI:3524957
Synonyms: 2xTg Ad, APdE9, APP-PS1, APP/PS1, APPswe/PS1dE9, APPswe/PS1deltaE9, Mo/Hu APPswe PS1dE9
Transgene: Tg(APPswe,PSEN1dE9)85Dbo  Location: Chr9:112832728-112832729 bp  Genetic Position: Chr9, Syntenic
Beta-amyloid plaques in Tg(APPswe,PSEN1dE9)85Dbo/0 mice and neurofibrillary tangles in Mapttm1(EGFP)Klt/Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/0 mice

Show the 1 phenotype image(s) involving this allele.

Strain of Origin:  (C57BL/6 x C3H)F2
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutations:    Duplication, Insertion
Tg(APPswe,PSEN1dE9)85Dbo expresses 2 genes
Mutation detailsTwo transgenes inserted at a single locus in Chromosome 9 between Arpp21 and Pdcd6ip. Each transgene is controlled by the mouse prion promoter and contains a cDNA sequence. In one transgene the cDNA encodes a chimeric amyloid beta (A4) precursor protein (APPswe). In the second transgene the cDNA encodes the "DeltaE9" mutation of human presenilin 1. The DeltaE9 mutation of the human presenilin 1 gene is a deletion of exon 9 and corresponds to a form of early-onset Alzheimer's disease. The amyloid beta precursor protein coding sequences were altered by replacing mouse sequence encoding three amino acids of the A-beta domain with the human coding sequence for these residues. The chimeric amyloid beta (A4) precursor protein sequence was then further modified to encode the Swedish mutations K595N/M596L found in human. Both the transgenic peptide and holoprotein are detected by Signet Laboratories' monoclonal 6E10 antibody, which is specific for human sequence within this region. Human presenilin protein, which in high levels displaces detectable endogenous mouse protein, is immunodetected in the double transgenic mouse in whole brain protein homogenates. Human amyloid precursor protein is also immunodetected in these mice in whole brain protein homogenates. Transgene insertion occurred on Chr 9, causing a 1 bp duplication. (J:78664, J:227541)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 7 strains available      Cell Lines: 13 lines available
Mice carrying this double transgene develop beta-amyloid deposits in the brain by 6 to 7 months of age.
Original:  J:78664 Jankowsky JL, et al., Co-expression of multiple transgenes in mouse CNS: a comparison of strategies. Biomol Eng. 2001 Jun;17(6):157-65
All:  861 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.20
The Jackson Laboratory