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Nrp1tm1Ddg
Targeted Allele Detail
Nomenclature
Symbol: Nrp1tm1Ddg
Name: neuropilin 1; targeted mutation 1, David D Ginty
MGI ID: MGI:3512099
Synonyms: npn-1Sema-
Gene: Nrp1  Location: Chr8:128358604-128503363 bp, + strand  Genetic Position: Chr8, 75.78 cM, cytoband E
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:93799
Parent Cell Line:  GK129 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted
Mutation:    Insertion
 
Mutation detailsExon 2 was replaced with a mutated exon 2 encoding 7 amino acid substitutions (H46E, S47A, S51A, E52R, K53D, D79K, R80E) in three regions of the amino-terminal CUB domain, and a loxP site flanked neomycin resistance gene cassette was inserted into intron 1. The neo cassette was removed through subsequent cre-mediated recombination. Mutation of these sites was shown to abolish Sema3 family binding but not VEGF binding. Immunohistochemistry showed that homozygotes express normal levels of protein. (J:93799)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 26 assay results
In Structures Affected by this Mutation: 11 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Nrp1 Mutation:  47 strains or lines available
References
Original:  J:93799 Gu C, et al., Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development. Dev Cell. 2003 Jul;5(1):45-57
All:  34 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory