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Tg(Krt1-15-cre/PGR*)22Cot
Transgene Detail
Nomenclature
Symbol: Tg(Krt1-15-cre/PGR*)22Cot
Name: transgene insertion 22, George Cotsarelis
MGI ID: MGI:3511785
Synonyms: K15CrePR, K15-CrePR1, Krt15-cre/PGR)22Cot, Tg(Krt1-15-cre/PGR)22Cot
Transgene: Tg(Krt1-15-cre/PGR*)22Cot  Location: unknown  
Transgene
origin
Strain of Origin:  (C57BL/6J x SJL/J)F2
Transgene
description
Transgene Type:    Transgenic (Inducible, Recombinase)
Inducer:    mifepristone
Mutation:    Insertion
 
Mutation detailsThe transgene comprises the entire 5.0-kb mouse Krt1-15 promoter region driving expression of a fusion protein composed of an amino-terminal nuclear localization signal followed by Cre recombinase joined to the carboxyl-terminal hormone binding domain (HBD) of a mutant human progesterone receptor. This mutant HBD, whose carboxyl terminal is truncated by 42 amino acids, fails to bind endogenous progestins but binds and, paradoxically, is activated by progesterone receptor antagonists such as RU486. Constitutive expression of the fusion protein is directed by the Krt1-15 promoter to epithelial stem cells in the bulge region of the hair follicle. It remains inactive in the cytoplasm until bound to RU486, whereupon it translocates to the nucleus and can catalyze loxP-mediated recombination. (J:84610, J:94076, J:94082)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Krt15     Summary of all recombinase alleles driven by Krt15. Comparative matrix view of recombinase activities
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Notes
This transgenic line is one of seven founder lines expressing the transgene, as assessed by RT-PCR. Mice of each line were crossed to mice expressing a beta-galactosidase Cre reporter transgene. Following topical application of RU486, bitransgenic mice of lines 22, 42 and 49 were shown to exhibit beta-galactosidase activity in their skin. Line 22, which showed the least enzyme activity in untreated skin, was selected for further analysis.
References
Original:  J:94076 Morris RJ, et al., Capturing and profiling adult hair follicle stem cells. Nat Biotechnol. 2004 Apr;22(4):411-7
All:  62 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory