Tg(CAG-EYFP)YC5Nagy
Transgene Detail

Summary

• Symbol: Tg(CAG-EYFP)YC5Nagy
• Name: transgene insertion YC5, Andras Nagy
• MGI ID: MGI:3497940
• Synonyms: Tg(ActbEYFP)1Nagy, Tg(ACTB-EYFP)1Nagy, Tg(ACTB-EYFP)YC5Nagy, Tg(CAG-EYFP)YC5Nagy, YC5/EYFP
• Transgene: Tg(CAG-EYFP)YC5Nagy Location: unknown
• Alliance: Tg(CAG-EYFP)YC5Nagy page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:64336
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene contains an Enhanced Yellow Fluorescent Protein gene under the control of the a chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer. (J:64336)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Homozygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. All tissues from hemizygous animals display fluorescence in all cell types under appropriate lighting conditions. Homozygotes fluoresce with twice the intensity of the hemizygotes. Notable exceptions to this phenotype are erythrocytes and adipocytes in which fluorescence is negligible or absent.

References

• Original: J:64336 Hadjantonakis AK, et al., FACS for the isolation of individual cells from transgenic mice harboring a fluorescent protein reporter. Genesis. 2000 Jul;27(3):95-8
• All: 2 reference(s)