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Gfi1tm2Tmo
Targeted Allele Detail
Nomenclature
Symbol: Gfi1tm2Tmo
Name: growth factor independent 1 transcription repressor; targeted mutation 2, Tarik Moroy
MGI ID: MGI:3057195
Synonyms: GfiGFP, GfiKI
Gene: Gfi1  Location: Chr5:107864521-107873671 bp, - strand  Genetic Position: Chr5, 52.23 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:93337
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA targeting vector was designed to replace exons 3-5 with an in-frame GFP and insert a floxed neo. Crossing with mice expressing Cre recombinase in the germ line removed the neomycin resistance gene. Fluorescence was detectible in virtually all thymocytes of mutant mice. Western blot showed that GFP expression in mutants was similar to that of gene expressed in wild-type mice. (J:93337)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 11 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gfi1 Mutation:  19 strains or lines available
References
Original:  J:93337 Yucel R, et al., Gfi1:green fluorescent protein knock-in mutant reveals differential expression and autoregulation of the growth factor independence 1 (Gfi1) gene during lymphocyte development. J Biol Chem. 2004 Oct 24;279(39):40906-17
All:  24 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory