Tg(Pcp2-EGFP)146.244Yuza
Transgene Detail

Summary

• Symbol: Tg(Pcp2-EGFP)146.244Yuza
• Name: transgene insertion 146.244, Michisuke Yuzaki
• MGI ID: MGI:3057131
• Synonyms: L7-GFP
• Transgene: Tg(Pcp2-EGFP)146.244Yuza Location: unknown
• Alliance: Tg(Pcp2-EGFP)146.244Yuza page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene contains an Enhanced Green Fluorescent Protein gene under the control of the mouse Purkinje cell protein 2 promoter. (J:93484)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These mice display distinct expression of GFP in dendrites, axons and soma of Purkinje cells, allowing identification, isolation, and purification of Purkinje cells by FACS. GFP expression mimics endogenous Purkinje cell protein 2 expression pattern. Fluorescence is detectable at embryonic day 17 in Purkinje cells and also occurs in retinal bipolar cells. Low levels of fluorescence are seen in olfactory periglomerular cells, interpeduncular nucleus, and superior colliculus neurons.

References

• Original: J:93484 Tomomura M, et al., Purification of Purkinje cells by fluorescence-activated cell sorting from transgenic mice that express green fluorescent protein. Eur J Neurosci. 2001 Jul;14(1):57-63
• All: 2 reference(s)