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Tg(TcraTcrb)1100Mjb
Transgene Detail
Nomenclature
Symbol: Tg(TcraTcrb)1100Mjb
Name: transgene insertion 1100, Michael J Bevan
MGI ID: MGI:3054907
Synonyms: OT1, OT-1, OT1 TCR, OTI, OT-I, OT-I(OVA)TCR, OT-ITCR, OT-I TCR, OVA-tcr-I, OVA-Tg, TCR-OT-I, Tg(OT-I), Tg(TcrOva)1100Mjb, Tg(TcrOVA)1100 mjb
Transgene: Tg(TcraTcrb)1100Mjb  Location: unknown  Genetic Position: Chr3, Syntenic
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(TcraTcrb)1100Mjb expresses 2 genes
 
Mutation detailsTransgenic mice carry two coinjected constructs containing rearranged mouse TCR alpha (Tcra-V2, Tcra-J(alpha26)) and TCR beta (Tcrb-V5, Tcrb-D(beta2), Tcrb-J(beta2.6)) chains. This transgenic T cell receptor recognizes ovalbumin residues 257-264 (SIINFEKL) in the context of H2Kb. The rearranged Tcra (alpha chain) cDNA specific for ovalbumin was isolated from T cells (clone AD10) and is expressed under the control of a 2-kb fragment of the H-2Kb promoter, a 700-bp fragment including the Ig H chain enhancer located 5' to the promoter, and a 1.7-kb BamHI-PstI fragment containing the 3' half of the human adult 8-globin gene (including part of exon 2, the exon 2-exon 3 C.F6 TCR a-chain gene that was truncated at the 3' XbaI site intron, exon 3) and polyadenylation signal. The Tcrb (beta chain) construct consists of a genomic fragment containing the Tcrb coding and endogenous promoter regions from a T cell clone (B3) specific for ovalbumin. (J:92867)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 5 strains available      Cell Lines: 0 lines available
Notes
Phenotypic Similarity to Human Syndrome: Anaplastic Large Cell Lymphoma J: 235890 in double Tg(Cd4-NPM/ALK)N1Ingh and Tg(TcraTcrb)1100Mjb mice.
References
Original:  J:92867 Hogquist KA, et al., T cell receptor antagonist peptides induce positive selection. Cell. 1994 Jan 14;76(1):17-27
All:  1166 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory