Tg(tetop-lacZ)2Mam
Transgene Detail

Summary

• Symbol: Tg(tetop-lacZ)2Mam
• Name: transgene insertion 2, Lothar Hennighausen
• MGI ID: MGI:3053973
• Synonyms: TetO-beta-gal, tTAluc
• Transgene: Tg(tetop-lacZ)2Mam Location: unknown
• Alliance: Tg(tetop-lacZ)2Mam page

Transgene origin

• Strain of Origin: C57BL/6 x SJL

Transgene description

• Transgene Type: Transgenic (Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The construct contains a nuclear localization signal, the beta-galactosidase structural gene, an intron, and a poly(A) signal. Expression of beta-galactosidase is controlled by a tetracycline-responsive promoter element (TRE) consisting of the human cytomegalovirus early promoter fused to tet operator sequences. No beta-galactosidase activity was observed in the absence of a tTA transgene. (J:92587)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Four lines were produced (G1-G4).

In mice carrying both this transgene and a transgene expressing tTA or rtTA, lacZ transcription may be induced by the administration (or withdrawal, if tTA is used) of tetracycline, or its analog, doxycyline. No beta-galactosidase activity is observed in the absence of a tTA transgene.

References

• Original: J:92587 Furth PA, et al., Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9302-6
• All: 15 reference(s)