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Transgene Detail
Symbol: Tg(tetop-lacZ)2Mam
Name: transgene insertion 2, Lothar Hennighausen
MGI ID: MGI:3053973
Synonyms: TetO-beta-gal, tTAluc
Transgene: Tg(tetop-lacZ)2Mam  Location: unknown  
Strain of Origin:  C57BL/6 x SJL
Transgene Type:    Transgenic (Inducible, Reporter)
Mutation:    Insertion
Mutation detailsThe construct contains a nuclear localization signal, the beta-galactosidase structural gene, an intron, and a poly(A) signal. Expression of beta-galactosidase is controlled by a tetracycline-responsive promoter element (TRE) consisting of the human cytomegalovirus early promoter fused to tet operator sequences. No beta-galactosidase activity was observed in the absence of a tTA transgene. (J:92587)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Four lines were produced (G1-G4).

In mice carrying both this transgene and a transgene expressing tTA or rtTA, lacZ transcription may be induced by the administration (or withdrawal, if tTA is used) of tetracycline, or its analog, doxycyline. No beta-galactosidase activity is observed in the absence of a tTA transgene.

Original:  J:92587 Furth PA, et al., Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9302-6
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.15
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