Tg(pPWL88puro)2Ems
Transgene Detail

Nomenclature

• Symbol: Tg(pPWL88puro)2Ems
• Name: transgene insertion 2, Elizabeth M Simpson
• MGI ID: MGI:3052971
• Transgene: Tg(pPWL88puro)2Ems Location: unknown

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgene contains a phosphoglycerate kinase 1 promoter and the bacterial puromycin resistance gene, puromycin N-acetyltransferase (pac). (J:92461)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Embryonic fibroblasts derived from transgenic mice on a C57BL/6 background are resistant to 2 ug/ml puromycin. Homozygous transgenic embryos die before birth.

References

• Original: J:92461 Linnell ER, et al., Transgenic mice for the preparation of puromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selection. Mamm Genome. 2001 Feb;12(2):169-71
• All: 1 reference(s)