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Tg(pPWL88puro)2Ems
Transgene Detail
Nomenclature
Symbol: Tg(pPWL88puro)2Ems
Name: transgene insertion 2, Elizabeth M Simpson
MGI ID: MGI:3052971
Transgene: Tg(pPWL88puro)2Ems  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6J
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Mutation detailsThe transgene contains a phosphoglycerate kinase 1 promoter and the bacterial puromycin resistance gene, puromycin N-acetyltransferase (pac). (J:92461)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
Embryonic fibroblasts derived from transgenic mice on a C57BL/6 background are resistant to 2 ug/ml puromycin. Homozygous transgenic embryos die before birth.
References
Original:  J:92461 Linnell ER, et al., Transgenic mice for the preparation of puromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selection. Mamm Genome. 2001 Feb;12(2):169-71
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
03/30/2021
MGI 6.16
The Jackson Laboratory