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Transgene Detail
Symbol: Tg(pPWL512hyg)1Ems
Name: transgene insertion 1, Elizabeth M Simpson
MGI ID: MGI:3052969
Transgene: Tg(pPWL512hyg)1Ems  Location: unknown  
Strain of Origin:  C57BL/6J
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
    The transgene contains a PGK promoter and the bacterial hygromycin resistance gene. (J:92459)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Transgene also called "feeder" and "hyg."

Transgenic mice on a C57BL/6 background express the hygromycin resistance gene in a wide variety of tissues including muscle. Embryonic feeder layers derived from these mice are resistant to up to 0.8mg/ml hygromycin B, whereas feeder layers from normal C57BL/6J mice are killed by 0.2mg/ml hygromycin B.

Mouse embryonic fibroblasts prepared from homozygous transgenic mice that are also homozygous for Tg(Hprt1)1Jae and Dnmt1tm3Jae are resistant to concentrations of the drugs G418, 6TG, puromycin, and hygromycin well above those used normally for the selection of drug-resistant ES cells.

Original:  J:92459 Johnson KA, et al., Transgenic mice for the preparation of hygromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selections. Nucleic Acids Res. 1995 Apr 11;23(7):1273-5
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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