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Transgene Detail
Symbol: Tg(MTn-lacZ)204Bri
Name: transgene insertion 204, Ralph L Brinster
MGI ID: MGI:3052912
Transgene: Tg(MTn-lacZ)204Bri  Location: unknown  
Strain of Origin:  C57BL/6J
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
Mutation detailsThe mouse Mt1 (metallothionein 1) promoter and flanking DNA (-588 to +68) were fused to the bacterial lacZ gene with a nuclear localization sequence. The construct was inserted into the EcoRI site of a vector that carries 10kb of sequence flanking Mt2 and 7kb flanking the Mt1 gene. These flanking sequences contain ribonuclease-hypersensitive sites thought to be important for enhanced expression. (J:92451)
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Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Transgenic mice on a C57BL/6J background display no obvious phenotype. The protein product is localized to the cell nucleus. Expression is present in the liver of transgenic mice as a result of the presence of the SV40 large T-antigen nuclear localization signal peptide. Hepatocytes stain blue with X-gal staining. Expression can be induced up to 10-fold by administration of heavy metal ions.
Original:  J:92451 Rhim JA, et al., Replacement of diseased mouse liver by hepatic cell transplantation. Science. 1994 Feb 25;263(5150):1149-52
All:  3 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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