Mtor^tm1Yam
Targeted Allele Detail

Summary

• Symbol: Mtor^tm1Yam
• Name: mechanistic target of rapamycin kinase; targeted mutation 1, Shinya Yamanaka
• MGI ID: MGI:3052669
• Synonyms: mTOR^-
• Gene: Mtor Location: Chr4:148533068-148642140 bp, + strand Genetic Position: Chr4, 78.76 cM, cytoband E1
• Alliance: Mtor^tm1Yam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92252
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 44 to 47, encoding the kinase domain, were replaced by an IRES-Betageo-pA cassette. Northern blot of heterozygous ES cells showed reduced expression and detected a larger transcript, corresponding to the fusion of Frap1 to Betageo. Western blot showed that protein levels were also reduced. (J:92252)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mtor Mutation: 115 strains or lines available

References

• Original: J:92252 Murakami M, et al., mTOR is essential for growth and proliferation in early mouse embryos and embryonic stem cells. Mol Cell Biol. 2004 Aug;24(15):6710-8
• All: 1 reference(s)