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Rasd2tm1Rdl
Targeted Allele Detail
Nomenclature
Symbol: Rasd2tm1Rdl
Name: RASD family, member 2; targeted mutation 1, Roberto Di Lauro
MGI ID: MGI:3050759
Synonyms: Rhes-
Gene: Rasd2  Location: Chr8:75213944-75224113 bp, + strand  Genetic Position: Chr8, 35.61 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:91597
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsA targeting vector was used to insert a stop codon in each frame immediately downstream of the endogenous translational start codon. An IRES-EGFP was placed downstream of the endogenous regulatory sequences and a floxed PGKneo were also inserted. Southern blot confirmed recombination. In situ hybridization showed that mRNA was barely detectable in the striatal region of mutant brains. In situ hybridization using an antisense riboprobe of EGFP shows that EGFP mRNA was detected in mutants. Western blotting of striatum protein extracts showed putatively nonspecific bands that were present in both wild-type and knockouts. Therefore, the mutated allele produces no detectable Rasd2 protein. (J:91597)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Rasd2 Mutation:  6 strains or lines available
References
Original:  J:91597 Spano D, et al., Rhes is involved in striatal function. Mol Cell Biol. 2004 Jul;24(13):5788-96
All:  9 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
08/23/2016
MGI 6.05
The Jackson Laboratory