Kif18a^gcd2
Chemically induced Allele Detail

Summary

• Symbol: Kif18a^gcd2
• Name: kinesin family member 18A; germ cell depletion, John Schimenti
• MGI ID: MGI:3050507
• Gene: Kif18a Location: Chr2:109111165-109172094 bp, + strand Genetic Position: Chr2, 56.55 cM
• Alliance: Kif18a^gcd2 page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86161
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Chemically induced (other) (Null/knockout)
• Mutation: Single point mutation
• Mutation details: The gcd2 mutation affects the highly conserved R308 residue within the KIF18A motor domain. The gcd2 mutation is a G-to-A transition in exon 7. This is a missense mutation that changes an AGA codon to an AAA, leading to the single amino acid change, R308K. Arginine 308 is a highly conserved amino acid in the motor domain of the protein. (J:222418)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:	36 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Kif18a Mutation: 63 strains or lines available

Notes

ES cells were mutagenized using ethyl methansulfonate (EMS). Generation 3 mice on a mixed 129S1/SvImJ and C57BL/6 background were identified by examining ductus deferens sperm for defects in morphology, motility, and quantity.

References

• Original: J:86161 Ward JO, et al., Toward the genetics of mammalian reproduction: induction and mapping of gametogenesis mutants in mice. Biol Reprod. 2003 Nov;69(5):1615-25
• All: 5 reference(s)