About   Help   FAQ
Prnp/Prndtm1Aag
Targeted Allele Detail
Nomenclature
Symbol: Prnp/Prndtm1Aag
Name: prion like protein doppel; targeted mutation 1, Adriano Aguzzi
MGI ID: MGI:3047634
Synonyms: Prno
Gene: Prnd  Location: Chr2:131950861-131956130 bp, + strand  Genetic Position: Chr2, 64.07 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:89232
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intergenic deletion
 
Mutation detailsUsing transalleleic targeted meiotic recombination, this allele was created in which both the Prnd and Prnp loci are disrupted. The recombined allele lacks ~16 kb encompassing both genes. To generate this allele, Prnptm2.1Cwe mice, which have a loxP site in place of the coding sequence, were crossed with Prndtm1Aag mice, which have a floxed neo in place of the coding exon. Northern blot analysis indicated absence of Prnp and Prnd transcript in brain, spleen and testes of mutants. (J:89232)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Prnd Mutation:  4 strains or lines available
References
Original:  J:89232 Genoud N, et al., Disruption of Doppel prevents neurodegeneration in mice with extensive Prnp deletions. Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4198-203
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
06/11/2019
MGI 6.14
The Jackson Laboratory