Capn3^{Gt(OST141731)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Capn3^{Gt(OST141731)Lex}
• Name: calpain 3; gene trap OST141731, Lexicon Genetics
• MGI ID: MGI:3046866
• Synonyms: C3KO, Capn3^{Gt(neo)453Lex}, Capn3^{Gt(neo-btk)1Spc}, Capn3-^tm1Spc
• Gene: Capn3 Location: Chr2:120294074-120335400 bp, + strand Genetic Position: Chr2, 60.31 cM
• Alliance: Capn3^{Gt(OST141731)Lex} page

Mutation origin

• Mutant Cell Line: OST141731
• Germline Transmission: Earliest citation of germline transmission: J:91206
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: A gene trap vector containing a neo-btk disrupted the gene by integrating into intron 1, thereby introducing premature stop codons after transcription. No gene products were detected by RT-PCR, including any splice variants. Western blot confirmed absence of full-length protein and fusion proteins of any size. (J:91206)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST141731	CG544481 ; (MGI Seq Detail)	3' RACE	Chr2:120312612-120315830 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  1 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Capn3 Mutation: 48 strains or lines available

References

• Original: J:91206 Kramerova I, et al., Null mutation of calpain 3 (p94) in mice causes abnormal sarcomere formation in vivo and in vitro. Hum Mol Genet. 2004 Jul 1;13(13):1373-88
• All: 12 reference(s)