Igh^tm6Cgn
Targeted Allele Detail

Summary

• Symbol: Igh^tm6Cgn
• Name: immunoglobulin heavy chain complex; targeted mutation 6, University of Cologne
• MGI ID: MGI:3046857
• Synonyms: D42H
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76117
• Parent Cell Line: BL/6-III (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The DQ52 element of Igh-D was disrupted by the insertion of a floxed neo cassette and the Igh-J elements were replaced with sequence encoding the variable region of DNA binding antibody D42. In constrast to Igh^tm3Cgn, this allele contains Igh-V11 mutations resulting in a Ser to Asn substitution at position 35 in CDR1 and an Asn to Tyr substitution at position 53 in CDR2. The Igh intron enhancer was left intact. Flow cytometry verified cell surface expression of the mutant protein product. The floxed neo cassette was removed via cre-mediated recombination. (J:76117)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 41 strains or lines available

References

• Original: J:76117 Pewzner-Jung Y, et al., B cell deletion, anergy, and receptor editing in 'knock in' mice targeted with a germline-encoded or somatically mutated anti-DNA heavy chain. J Immunol. 1998 Nov 1;161(9):4634-45
• All: 5 reference(s)