Ikbke^tm1Aki
Targeted Allele Detail

Summary

• Symbol: Ikbke^tm1Aki
• Name: inhibitor of kappaB kinase epsilon; targeted mutation 1, Shizuo Akira
• MGI ID: MGI:3046788
• Synonyms: Ikk-I^-
• Gene: Ikbke Location: Chr1:131182337-131207339 bp, - strand Genetic Position: Chr1, 56.93 cM
• Alliance: Ikbke^tm1Aki page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91363
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo cassette replaced exons 7 and 8, which encode part of the kinase domain. Sequencing of mRNA from the mutant allele confirmed lack of exons 7 and 8 and indicated a premature stop codon was created, thereby generating a truncated protein that contained only a part of the kinase domain. A transfection assay showed that mutant protein did not function as the wild-type. (J:91363)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ikbke Mutation: 55 strains or lines available

References

• Original: J:91363 Hemmi H, et al., The Roles of Two IkappaB Kinase-related Kinases in Lipopolysaccharide and Double Stranded RNA Signaling and Viral Infection. J Exp Med. 2004 Jun 21;199(12):1641-50
• All: 14 reference(s)