Rho^{tm3(RHO/GFP)Jhw}
Targeted Allele Detail

Summary

• Symbol: Rho^{tm3(RHO/GFP)Jhw}
• Name: rhodopsin; targeted mutation 3, John H Wilson
• MGI ID: MGI:3046187
• Synonyms: hrhoG(H)
• Gene: Rho Location: Chr6:115908709-115916997 bp, + strand Genetic Position: Chr6, 53.72 cM
• Alliance: Rho^{tm3(RHO/GFP)Jhw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91038
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The endogenous gene was first replaced with an HPRT minigene flanked by loxH and lox511 and then the HPRT minigene was replaced with the human RHO gene fused to GFP via cre mediated recombination. This resulted in replacement of the endogenous gene by human RHO fused to GFP flanked by loxH and lox511 sites. Western blot analysis indicated that expression of the human gene was about 16% that of the endogenous gene. GFP expression is seen in the eyes. (J:91038)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rho Mutation: 48 strains or lines available

References

• Original: J:91038 Chan F, et al., Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy. Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):9109-14
• All: 1 reference(s)