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Targeted Allele Detail
Symbol: Chattm1.1Jrs
Name: choline acetyltransferase; targeted mutation 1.1, Joshua R Sanes
MGI ID: MGI:3045900
Synonyms: ChAT-
Gene: Chat  Location: Chr14:32408203-32465909 bp, - strand  Genetic Position: Chr14, 19.4 cM
Germline Transmission:  Earliest citation of germline transmission: J:91066
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsChattm1Jrs-carring mice were crossed with transgenic mice expressing cre in the germ line to produce this allele in which exons 3 and 4 were replaced with an frt-flanked neo. Southern blot confirmed excision of the exons. RT-PCR revealed the splicing of exon 2 to exon 5, resulting in a frame shift after the codon for amino acid 22 and a stop codon at amino acid 31. Therefore, the protein products were less than 32 amino acids long, instead of the 641, and lacked the catalytic center. (J:91066)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Chat Mutation:  19 strains or lines available
Original:  J:91066 Misgeld T, et al., Roles of neurotransmitter in synapse formation: development of neuromuscular junctions lacking choline acetyltransferase. Neuron. 2002 Nov 14;36(4):635-48
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory