Rfx3^tm1.1Wrth
Targeted Allele Detail

Summary

• Symbol: Rfx3^tm1.1Wrth
• Name: regulatory factor X, 3 (influences HLA class II expression); targeted mutation 1.1, W Reith
• MGI ID: MGI:3045792
• Synonyms: Rfx3^neoR
• Gene: Rfx3 Location: Chr19:27739121-27988566 bp, - strand Genetic Position: Chr19, 22.36 cM, cytoband C1
• Alliance: Rfx3^tm1.1Wrth page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89880
• Parent Cell Line: ENS (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Cre-mediated recombination of Rfx3^tm1Wrth resulted in the excision of floxed exon 3, leaving an FRT flanked neo. Exon 3 encodes the DNA binding domain. RT-PCR showed that the deletion of exon 3 resulted in the splicing of exon 2 to exon 4, leading to a frameshift and premature termination at an out-of-frame stop codon. (J:89880)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rfx3 Mutation: 68 strains or lines available

References

• Original: J:89880 Bonnafe E, et al., The transcription factor RFX3 directs nodal cilium development and left-right asymmetry specification. Mol Cell Biol. 2004 May;24(10):4417-27
• All: 2 reference(s)