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Spi1tm1Kast
Targeted Allele Detail
Nomenclature
Symbol: Spi1tm1Kast
Name: spleen focus forming virus (SFFV) proviral integration oncogene; targeted mutation 1, Philippe Kastner
MGI ID: MGI:3044765
Synonyms: PU.1G, PU-1-GFP
Gene: Spi1  Location: Chr2:90912750-90946104 bp, + strand  Genetic Position: Chr2, 50.44 cM, cytoband E3
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:90543
Parent Cell Line:  P1 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA 22 bp segment of exon 1 was replaced with a rabbit beta globin intron, an enhanced GFP sequence and an SV40 polyadenylation signal via homologous recombination. Both mRNA and protein were undetectable in fetal liver from homozygous mice. GFP expression correlated well with endogenous Sfpi1 expression. (J:90543)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 14 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Spi1 Mutation:  22 strains or lines available
References
Original:  J:90543 Back J, et al., PU.1 determines the self-renewal capacity of erythroid progenitor cells. Blood. 2004 May 15;103(10):3615-23
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/16/2021
MGI 6.17
The Jackson Laboratory