Tg(Pax4-lacZ)1Pgr
Transgene Detail

Summary

• Symbol: Tg(Pax4-lacZ)1Pgr
• Name: transgene insertion 1, Peter Gruss
• MGI ID: MGI:3044158
• Synonyms: CBII, CBIID, (Pax4-LacZ)Tg, Pax4 prom./beta-galactosidase
• Transgene: Tg(Pax4-lacZ)1Pgr Location: unknown
• Alliance: Tg(Pax4-lacZ)1Pgr page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: A 7.8-kb BALB/c genomic DNA fragment extending downstream from exon 3 of paired box gene 4 (Pax4) was ligated in-frame to the 5' end of the beta-galactosidase (lacZ) gene. Transgenic embryos express beta-galactosidase in the endocrine pancreas in recapitulation of the cell type- and developmental stage-specific expression of paired box gene 4 (Pax4). The genomic fragment includes a 407 base pair segment that in the genome resides approximately 1.3 kb upstream of the Pax4 transcription start site and that is 88% identical to the orthologous segment from Homo sapiens. This highly conserved region contains target sequences for four transcription factors that regulate pancreatic beta and delta cell-specific gene expression. (J:66530)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:66530 Brink C, et al., Pax4 regulatory elements mediate beta cell specific expression in the pancreas. Mech Dev. 2001 Jan;100(1):37-43
• All: 1 reference(s)