Actc1^tm1.1Buck
Targeted Allele Detail

Summary

• Symbol: Actc1^tm1.1Buck
• Name: actin, alpha, cardiac muscle 1; targeted mutation 1.1, Margaret E Buckingham
• MGI ID: MGI:3029177
• Synonyms: alphac-actin^nlaacZ1.1, alphac-actin^{nlaacZ1.1delta}
• Gene: Actc1 Location: Chr2:113877763-113883356 bp, - strand Genetic Position: Chr2, 57.55 cM
• Alliance: Actc1^tm1.1Buck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84604
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Sequence encoding mutated nuclear localized lacZ with intragenic duplication corresponding to nucleotides 870-1987 after the ATG such that a shift in codon phase introduces a TGA STOP codon at nucleotide 1993 was inserted immediately after the start codon via homologous recombination. A floxed neo cassette included in the targeting vector was excised via cre-mediated recombination. The expression pattern of lacZ recapitulated that of the endogenous gene. (J:84604)

Expression

In Mice Carrying this Mutation:

23 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actc1 Mutation: 24 strains or lines available

References

• Original: J:84604 Meilhac SM, et al., A retrospective clonal analysis of the myocardium reveals two phases of clonal growth in the developing mouse heart. Development. 2003 Aug;130(16):3877-89
• All: 4 reference(s)