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Targeted Allele Detail
Symbol: Bbc3tm1Ast
Name: BCL2 binding component 3; targeted mutation 1, Andreas Strasser
MGI ID: MGI:2681654
Synonyms: puma-
Gene: Bbc3  Location: Chr7:16308393-16318205 bp, + strand  Genetic Position: Chr7, 8.85 cM
Germline Transmission:  Earliest citation of germline transmission: J:86466
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsExons 2 and 3 which contain the BH3 interaction domain were replaced by a floxed PGK-neomycin expression cassette. The resistance cassette was removed in vivo by crossing mice to a C57BL/6 Deleter mouse line. Gene disruption was confirmed by RT-PCR and Northern blot analysis. (J:86466)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Bbc3 Mutation:  15 strains or lines available
Original:  J:86466 Villunger A, et al., p53- and drug-induced apoptotic responses mediated by BH3-only proteins puma and noxa. Science. 2003 Nov 7;302(5647):1036-8
All:  64 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
The Jackson Laboratory