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Targeted Allele Detail
Symbol: Sirt1tm1Fwa
Name: sirtuin 1; targeted mutation 1, Frederick W Alt
MGI ID: MGI:2677354
Synonyms: SIRT1deltaex4
Gene: Sirt1  Location: Chr10:63319005-63339035 bp, - strand  Genetic Position: Chr10, 32.57 cM
Developmental abnormalities in Sirt1tm1Fwa/Sirt1tm1Fwa mice.

Show the 1 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:85542
Parent Cell Line:  TC1/TC-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA floxed neomycin resistance cassette in reverse orientation was placed at the 3' end of intron 3. An additional loxP site was inserted just downstream of exon 4. In the resulting ES cells, exon 4, which encodes 51 amino acid residues of the conserved catalytic domain, is flanked by loxP sites. An AdenoCre construct was used to remove the neomycin selection cassette and exon 4. Corresponding protein was detected by Western blot analysis. These mutant ES cells, prior to cre recombination, were also used in the generation of Sirt1tm3Fwa mice. (J:85542)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 12 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Sirt1 Mutation:  51 strains or lines available
Original:  J:85542 Cheng HL, et al., Developmental defects and p53 hyperacetylation in Sir2 homolog (SIRT1)-deficient mice. Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9
All:  17 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory