Esr2^tm1Psh
Targeted Allele Detail

Summary

• Symbol: Esr2^tm1Psh
• Name: estrogen receptor 2 (beta); targeted mutation 1, Paul J Shughrue
• MGI ID: MGI:2676193
• Synonyms: ERBeta(-), ERBetaKO
• Gene: Esr2 Location: Chr12:76167193-76224033 bp, - strand Genetic Position: Chr12, 33.52 cM, cytoband D1-D3
• Alliance: Esr2^tm1Psh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76322
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 2 kb of genomic sequence encompassing portions of exons 1 and 2 as well as the intervening intron were replaced with a cassette containing translational stop codons in all three reading frames, followed by a floxed neomycin resistance gene. The absence of protein was confirmed by Western blot analysis and by immunocytochemistry. (J:76322)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Esr2 Mutation: 32 strains or lines available

References

• Original: J:76322 Shughrue PJ, et al., Estrogen-binding sites and their functional capacity in estrogen receptor double knockout mouse brain. Endocrinology. 2002 May;143(5):1643-50
• All: 5 reference(s)