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Targeted Allele Detail
Symbol: Mfge8tm1Shur
Name: milk fat globule-EGF factor 8 protein; targeted mutation 1, Barry D Shur
MGI ID: MGI:2675967
Synonyms: SED1-null
Gene: Mfge8  Location: Chr7:79133768-79149060 bp, - strand  Genetic Position: Chr7, 44.9 cM
Germline Transmission:  Earliest citation of germline transmission: J:85225
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA single loxP site remained in place of exon 1 after homologous recombination of flanking loxP sites and subsequent cre-mediated excision. Transcript was undetected in homozygous mutant mice by RT-PCR analysis of testicular cDNA using primers spanning exons 2 and 3 and exons 7 and 8. The absence of protein was indicated by Western blot analysis and by indirect immunofluorescence of live sperm. (J:85225)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mfge8 Mutation:  8 strains or lines available
Original:  J:85225 Ensslin MA, et al., Identification of mouse sperm SED1, a bimotif EGF repeat and discoidin-domain protein involved in sperm-egg binding. Cell. 2003 Aug 22;114(4):405-17
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory